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During the 16th century, Scotland became embroiled in the throes of the Protestant Reformation. John Knox stood out as the primary figure in this battle. A disciple of John Calvin, Knox, waged fierce battles with the forces of Catholic orthodoxy and eventually converted the country to Presbyterianism. Only the most distant parts of the Highlands retained a taste for older forms. Presbyterianism first gained a foothold in 1556 but it didn't really get going until John Knox's firebrand oratory started stirring people up, most notably in Perth, when he roused the townsfolk so much that a mob scoured the countryside for Catholics after one of his sermons, destroying the old abbey at Scone in the process. Within a few years Parliament had legislated for a National Presbyterian church, the Catholic Queen had fled, Scotland had Protestant England rather than Catholic France as its main ally, and firm Presbyterian tutors were in charge of the upbringing of the infant King.

Scotland has a civic culture somewhat distinct from that of the rest of the British Isles. It originates from various differences, some entrenched as part of the Act of Union, others facets of nationhood not readily defined but readily identifiable.

The written history of Scotland largely begins with the coming of the Roman empire to Britain. Although the pre-Roman inhabitants occasionally used writing for commemorative purpose, these societies favoured a strong oral history. With the loss of the druidic tradition (due to war, famine, and particularly the proscriptions of later Christian missionaries), the people forgot much of this lore. The only surviving pre-Roman account of Scotland originated with the Greek Pytheas of Massalia who circumnavigated the British islands (which he called PretThe British Saint Ninian conducted the first Christian mission in Scotland. From his base, the Candida Casa (present-day Whithorn) on the Solway Firth, he spread the faith in the south and east of Scotland and in the north of England. However, according to the writings of Saint Patrick and Saint Columba, the Picts appear to have renounced Christianity in the century between Ninian's death (432) and the arrival of Saint Columba in 563. The reason is not known. The Gaels re-introduced Christianity into Pictish Scotland, gradually pushing out worship of the older Celtic gods. The most famous evangelist of that period, Saint Columba, came to Scotland in 563 and settled on the island of Iona. Some consider his (possibly apocryphal) conversion of the Pictish King Brude the turning point in the Christianization of Scotland.

The largest political party operating in Scotland is the Labour Party. They replaced the Liberals as Scotland's main political force in the early twentieth century and traditionally represent the interests of workers and trade-unionists. They currently operate as the senior partners in a coalition Scottish Executive.

Church of England broke with Catholicism primarily for political reasons. Thus they replaced very little traditional Catholic theology, except to substitute the Crown for the Pope as the head of the Church. The Scots on the other hand were primarily Presbyterian, a movement which was the result of a strong theological rejection of certain Catholic teachings. In particular they were sceptical of the authority of the Pope and priesthood generally, which they rejected in favour of the priesthood of all believers. This doctrine was seen by both sides as radically undermining the authority not just of the priestly class, but of the aristocracy since it was essentially democratic.